Figure 6: Complementation with WT but not HDH ALKBH4 can rescue defects associated with cytokinesis failure.

(a) Quantification of apoptotic cells (MRC5) following treatment with CTRL or ALKBH4 siRNA for 48 h. Quantification is based on three independent experiments (30,000 cells per condition per experiment). P-values were calculated using a two-tailed t-test. Error bars indicate s.d. (b) MRC5 cells were simultaneously treated with CTRL or ALKBH4 siRNA and the indicated siRNA insensitive Flag-GFP-ALKBH4 constructs for 48 h then analysed by immunofluorecence with the indicated antibodies. Quantification is based on three independent experiments (300 cells per condition per experiment). P-values were calculated using a two-tailed t-test. Error bars indicate s.d. (c) As in (b). Quantification of anillin, NM II, Plk1 and aurora B, mislocalization following treatment with CTRL or ALKBH4 siRNA and the indicated siRNA insensitive Flag-GFP-ALKBH4. Quantification is based on three independent experiments (300 cells per condition per experiment). P-values were calculated using a two-tailed t-test. Error bars indicate s.d.