Figure 3: MARCO co-localizes with HSV-1 gC on the keratinocyte cell surface. | Nature Communications

Figure 3: MARCO co-localizes with HSV-1 gC on the keratinocyte cell surface.

From: HSV-1 exploits the innate immune scavenger receptor MARCO to enhance epithelial adsorption and infection

Figure 3

NHEK were incubated in the presence of purified HSV-1 gC for 2 h at 4 °C to allow binding of the glycoprotein to the cell surface. Unbound gC was removed by multiple wash steps before cells were fixed and incubated with both a mouse monoclonal antibody targeting HSV-1 gC, and rabbit polyclonal antibodies targeting MARCO (a), syndecan-1 (b), OLR1 (c) or SCARA3 (d). Physical proximity of MARCO, syndecan-1, OLR1 or SCARA3 to HSV-1 gC bound to the cell surface was determined using a fluorescence-based PLA that produces a red fluorescent signal only when the antigens recognized by the antibodies utilized in the assay reside within <40 nm of each other. Nuclei (blue) are stained with Hoescht. Scale bar, 100 μm. All data are from representative experiments repeated 2–4 times.

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