Figure 4: MARCO binds directly to HSV-1 gC.
Plate-bound purified MARCO protein was incubated with increasing concentrations of purified gC (a), gB (c) or gCΔC5/P (gC with a deletion of the heparin-binding domain, amino acids 33–123) (e). gC, gB and gCΔC5/P remaining bound after multiple wash steps was detected and quantified by ELISA. The dissociation constant (KD) and the maximum number of receptor-binding sites (Bmax) were determined using nonlinear regression with background subtracted using Graphpad Prism. In b and f, data in a and e, respectively, were transformed to create double-reciprocal plots to show linear-binding kinetics. (d) Plate-bound recombinant OLR 1 was incubated with purified gC. gC remaining bound after washing was detected and quantified by ELISA. (a–f) All individual replicate values plotted with offset overlapping points. All data are from representative experiments repeated at least two times.
