Figure 2: Spatial distributions of V-PspF under non-stress and stress conditions.
From: Dynamics and stoichiometry of a regulated enhancer-binding protein in live Escherichia coli cells
For SMI, we expressed stable and functional PspF (or its variants) from its native locus as an N-terminal fusion to fast maturing yellow fluorescent protein Venus (V-PspF) (see Supplementary Fig. S1A–C,E,F). E. coli MG1655 expressing chromosomal Venus-PspF (V-PspF) under control of its native promoter were imaged under (a) non-stress (psp off) and (b) pIV inducing stress (psp on) growth conditions and (c) cells expressing Venus-PspFW56A (V-PspFW56A) mutant were imaged under non-stress conditions. These proteins are shown as white foci (some marked with white arrow) within the cell (scale bar, 1 μM) in merged images of fluorescent and bright-field images of cells to illustrate of specific localizations. Graphs of (d) the number of V-PspF (n=314 for non-stress and n=197 for stress) or V-PspFW56A (n=185) foci per cell where x axis represents number of foci and y axis represents total percentage of cells, and (e) subcellular localizations of the foci on x axis (cartoon schematically presents the localization for the V-PspF foci studied) and percentage of cells (n=100 for non-stress, n=99 for stress and n=99 for V-PspFW56A) on y axis.
