Figure 2: Effects of epithelial lesioning on odour-evoked intrinsic optical images and odour recognition.

(a) Example scatter plots of ROI correlations for odour-evoked intrinsic signal activity maps recorded within sessions (session one, orange boxes; session two, yellow boxes) and across sessions (purple boxes) after sham treatment. Values for each ROI (open green circles) are expressed as normalized pixel value (NPV), r-values displayed are averages of all ROI correlations for each block comparison. The total number of data points plotted correspond to nROIs × pixels/ROI for one odour. Concomitant activity maps are displayed to the left of NPV correlations. NPV range, 0.9965–1.0027. Scale bar, 500 μm for all images. (b) Example scatter plots of ROI correlations (grey) for odour-evoked intrinsic signal activity maps within sessions (session one, orange boxes; session two, yellow boxes) and across sessions (purple boxes) after low-dose ZnSO₄ treatment. (c) Bar graph showing dissimilarity score of intrinsic signal activity maps (evoked by ethyl-butyrate and pentanal) from sham (n=27 activity maps, green open box) and low-dose-ZnSO₄-treated animals (n=32 activity maps, grey-filled box). NPV range, 0.996302−1.000254. Dissimilarity scores evaluated by Mann–Whitney U-test (*P<0.001). (d) Left, example odour-evoked activity maps from before (top, session one) and after (bottom, session two) nasal flush for sham-treated mice. Right, average dissimilarity score for pair B odour-evoked activity maps (left axis) and recognition scores for pair B odours (right axis) for shams. (e,f) As above for low- (e) and high-dose (f) ZnSO₄-treated mice. Data represent mean±s.e.m. (g) Recognition accuracy plotted against dissimilarity score for ethyl butyrate and pentanal from all mice in D1–D3. Pearson’s correlation value, P<0.05.