Figure 2: Rad51 binds in 5′–3′ direction.
From: Srs2 prevents Rad51 filament formation by repetitive motion on DNA
(a) Schematic of 3′ Cy3-labelled DNA used in PIFE assay. (b) Single-molecule trace displaying a gradual and stepwise increase in Cy3 signal. (c) Schematic of 5′-Cy3-labelled DNA used in PIFE assay. (d) Single-molecule trace showing an abrupt increase in Cy3 signal. (e) Normalized average intensity plots for both DNA constructs showing slower increase in 3′-Cy3 DNA compared with 5′-Cy3 DNA. Errors in fit results are in s.e.m. (f) Schematic summary of Rad51 binding in 5′–3′ direction.
