Figure 3: Srs2 exhibits repetitive motion on ssDNA.
From: Srs2 prevents Rad51 filament formation by repetitive motion on DNA
(a) Schematic diagram of FRET–DNA used in Srs2 assay. (b) Single-molecule traces displaying Srs2-induced FRET fluctuation. (c) The inverse of FRET peak-to-peak dwell time was fitted to Michaelis–Menten equation. Errors in fit results are in s.e.m. (d) Schematic diagram of tethered protein assay. Histidine (9 × )-tagged Srs2 was immobilized to surface and non-biotinylated FRET–DNA substrate was applied. (e) Single-molecule traces show that the repetitive motion arises from a single monomer of Srs2. DNA binding, Srs2-mediated repetitive motion and dissociation from Srs2 are shown as an appearance of FRET, FRET fluctuation and disappearance of FRET, respectively. (f) Single-molecule trace shows multiple bursts of repetitive events arising from single Srs2 monomer.
