Figure 4: Srs2 displays repetitive motion on short segments of ssDNA.
From: Srs2 prevents Rad51 filament formation by repetitive motion on DNA
(a) Schematic of FRET–DNA constructs with various tail lengths (T13–T70). (b) Single-molecule traces show similar range of FRET fluctuation in T13–T20, but much less prominent and lower FRET range fluctuation in T25–T30 and no fluctuation in T70. (c) FRET histograms of single-molecule traces of Srs2 on various ssDNA tail lengths. (d) FRET peak-to-peak dwell time δt. (e) Dwell times collected from various tail lengths. Error bars denote s.e.m. based on 500 molecules obtained from three independent measurements.
