Figure 5: Srs2 repetitive motion prevents Rad51 formation.
From: Srs2 prevents Rad51 filament formation by repetitive motion on DNA
(a) Schematic of FRET–DNA used in Rad51 removal assay. (b) Single-molecule traces showing low-to-high FRET followed by FRET fluctuation. (c) Single-molecule traces displaying dynamic exchange between Rad51 filament formation and Srs2 clearance. (d) Ratio between filament-formed duration and Srs2-cleared duration tested on varying tail. Error bars denote s.e.m. based on 100 events from 40 molecules. (e) Schematic of immobilized Srs2 assay to test whether clearance and repetitive activity arises from Srs2 monomer. (f) Single-molecule traces showing monomer Srs2 removing Rad51 filament and preventing re-formation. (g) Dwell times corresponding to removal duration for different tail length. Error bars denote s.e.m.
