Figure 6: Rad51 suppresses Srs2-induced unwinding.
From: Srs2 prevents Rad51 filament formation by repetitive motion on DNA
(a,b) FRET–DNA constructs with varying tail lengths (T13–T25) without and with Rad51. (c) Fluorescence spots left on surface can be counted for unwinding rate analysis. (d–f) Unwinding rate of Srs2 is substantially lower in the presence of Rad51. Error bars denote s.e.m. based on over 1,000 molecules obtained from three measurements. (g,h) Schematic of unwinding DNA constructs. Cy3 and Cy5 are located near duplex junction such that unwinding will result in FRET decrease. (i,j) Unwinding in the presence of Rad51 is delayed as indicated by slower FRET decrease. (k,l) Histograms and a Gaussian distribution fit show that the Srs2-unwinding duration is about 5–6 times longer in the presence of Rad51. Errors in fit results are in s.e.m.
