Figure 3: D14–SLR1 interaction in planta and the competition between the D14–SLR1 interaction and the GID1–SLR1 interaction.
From: Molecular mechanism of strigolactone perception by DWARF14
(a) BiFC analysis of the D14–SLR1 interaction. D14–GFP, cYFP–D14 and nYFP–SLR1 were expressed transiently in the protoplasts of Arabidopsis suspension-cultured cells. Scale bars, 10 μm. (b) Growth of AH109 transformants with pBridge-BDD14-MGID1 (or pBridge-BDD14) and pGAD-SLR1 on SD-His, Ade, Met plates with or without 10 μM (±)-GR24 and 1 μM GA4.When only (±)-GR24 was added to the growth medium, yeast colonies could grow independently of GID1 expression. In contrast, when both (±)-GR24 and GA3 were added, the GID1-expressing yeast could not grow on the SD-His, Ade and Met medium.
