Figure 5: Auxin fractionation after vacuole uptake.
From: Arabidopsis WAT1 is a vacuolar auxin transport facilitator required for auxin homoeostasis
Auxin is not converted within vacuoles. Isolated vacuoles were incubated in the presence of 14C-auxin (IAA) for 20 min and subsequently removed from the incubation medium as described for the transport studies. The water phase containing the vacuoles was subjected to a HPLC run, fractions were collected and the radioactivity present in each fraction was detected. A comparison of panel a, where non-radioactive auxin was detected at 29 min, with panel b representing the radioactivity detected in each fraction, shows that the radioactivity appears at a similar retention time as the authentic auxin. The slight shift is because of the collection of the fractions that occurred after the ultraviolet detector. WVL, wavelength. For more details see Methods.
