Figure 5: NEU1 loss-of-function in a model of AD accelerates β-amyloid production. | Nature Communications

Figure 5: NEU1 loss-of-function in a model of AD accelerates β-amyloid production.

From: Lysosomal NEU1 deficiency affects amyloid precursor protein levels and amyloid-β secretion via deregulated lysosomal exocytosis

Figure 5

(a,c) APP and β-amyloid levels, respectively, were analysed in hippocampal lysates from 5XFAD, 5XFAD/Neu1−/− and Neu1−/− hippocampi. (b,d) Quantification of a and c; the normalized levels of APP and β-amyloid were calculated by densitometric analysis of single bands on immunoblots probed with anti-APP or β-amyloid antibodies, and anti-tubulin in 5XFAD and in 5XFAD-Neu1−/− samples. Anti-tubulin antibody was used as loading controls; asterisks on graphic bars indicate statistically significant results, as determined by the Student’s t-test. Data are represented as the mean±s.d. (error bars); n=3 (e) Representative pictures of full-length APP staining in WT, 5XFAD, 5XFAD/Neu1−/− and Neu1−/− hippocampal regions (5XFAD, 5XFAD/Neu1−/− mice were 3 months of age, WT and Neu1−/− mice were 5 months of age; N-terminal antibody against APP). Scale bar, 20 μm.

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