Figure 2: Diverse dsRNA-binding proteins bind to a dsRNA source within the Caps locus.
From: RNA editing regulates transposon-mediated heterochromatic gene silencing
(a–c) Transgenic expression of dADAR-HA in polytene chromosomes not treated with RNAse in a, treated with Ribonuclease A (RNAse A) that cleaves single-stranded RNA in b or treated with Ribonuclease III (RNAse III) that cleaves dsRNA in c. (b) dADAR binding on the fourth chromosome is not abolished by RNAse A digestion (arrow). (c) RNAse III digestion results in the loss of dADAR binding on chromosome 4 (arrow) without abolishing nucleolar localization. (d) Transgenic Hydra magnipapillata ADAR-HA (HmADAR) driven by elav-Gal4 localizes to the Caps locus. (e) HmADAR transgene localization on a single polytene spread revealed numerous other binding locations (four examples shown) throughout the genome (arrows). (f) Elav-Gal4-driven B2-FLAG similarly localizes to the Caps locus. Arrows in a–c, and f point to prominent localization of dsRNA-binding proteins on the tip of chromosome 4. Scale bars, 20 μm.
