Figure 3: dADAR binds to dsRNA formed by inverted Hoppel elements.

(a) Schematic of the UAS-miniCAPS construct. (b) Co-localization of dADAR and Hoppel elements on chromosome 4 labelled using FISH. (c–f) dADAR does not bind to non-expressed pCasper[miniCAPS] in c, magnified in d but binds to UAS-miniCAPS driven by elav-Gal4 in e, magnified in f. Arrows point to miniCAPS insertions labelled via FISH for mini-white⁺. (g) Schematic of targeting construct used to delete Hoppel elements using homologous recombination. *Edited exon of Caps. (h) Expression of dADAR-HA in a Hok^mw/Hok⁺ background. FISH for mini-white⁺ (white) indicates deleted chromatid of chromosome 4, allowing visualization of the lack of dADAR binding to the Hok^mw chromatid. Chromosome 4 is denoted in each image. Scale bars, 20 μm. (i) Pie charts indicating editing levels at adenosines within the three Hoppel repeats. +/−signs in a and g denote the orientation of transcription.