Figure 3: Quantitative analysis of platelet FVIII. | Nature Communications

Figure 3: Quantitative analysis of platelet FVIII.

From: Platelet-targeted gene therapy with human factor VIII establishes haemostasis in dogs with haemophilia A

Figure 3

Flow cytometric analysis of platelets. BDDFVIII was absent in platelets (x axis) analysed from a FVIII-deficient dog used as a negative control for staining with a human BDDFVIII 1°Ab and Alexa Fluor 568-conjugated 2°Ab (black, unshaded histogram). In contrast G-PBC from transplanted dogs displayed appreciable levels of platelet BDDFVIII (shaded histogram: −889ITGA2B-driven FVIII of F20 and I42, yellow; −673ITGA2B-driven FVIII of M64, red). The hierarchy for the mean fluorescence intensity of FVIII expression reveals that F20<I42<M64. Shown are the results from one experiment analysis of 50,000 platelets per sample at 2.9 (F20), 1.9 (I42) and 0.9 (M64) years after infusion of FVIII-transduced G-PBC, which is representative of the outcome of seven separate experiments.

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