Table 1 Data collection and refinement statistics.
ZNRF3 ecto /RNF43 ecto | zZNRF3 | mZNRF3 | xZNRF3 | — | — | mZNRF3 | mZNRF3 | xZNRF3 | xRNF43 |
|---|---|---|---|---|---|---|---|---|---|
Rspo Fu1–Fu2 | — | — | — | xRSPO2 | xRSPO2–Pt | mRSPO2 | xRSPO2 | xRSPO2 | xRSPO2 |
Data collection | |||||||||
Space group | P21 | P21 | P21 | P41212 | P41212 | P212121 | P1 | P21 | C2 |
Cell dimensions | |||||||||
a, b, c (Å) | 36.0, 53.3, 72.5 | 47.3, 57.8, 50.5 | 49.2, 58.7, 52.7 | 97.1, 97.1, 292.9 | 96.6, 96.6, 290.1 | 59.8, 77.2, 130.6 | 36.4, 71.0, 72.0 | 56.0, 81.2, 71.6 | 88.9, 35.8, 87.9 |
α, β, γ (°) | 90, 102.1, 90 | 90, 97.6, 90 | 90, 93.6, 90 | 90, 90, 90 | 90, 90, 90 | 90, 90, 90 | 109.2, 101.7, 101.3 | 90, 113.0, 90 | 90, 114.6, 90 |
Resolution (Å)* | 42.59–1.60 (1.63–1.60) | 37.84–2.00 (2.05–2.00) | 37.66–2.40 (2.49–2.40) | 39.69–2.20 (2.25–2.20) | 39.46–3.20 (3.46–3.20) | 66.46–2.80 (2.97–2.80) | 38.89–2.40 (2.49–2.40) | 65.94–2.10 (2.16–2.10) | 32.76–2.70 (2.83–2.70) |
R merge | 0.074 (0.359) | 0.089 (0.353) | 0.067 (0.478) | 0.106 (0.852) | 0.218 (1.443) | 0.116 (1.345) | 0.073 (0.605) | 0.115 (0.788) | 0.147 (0.550) |
I/σI | 16.1 (2.3) | 9.7 (1.9) | 8.7 (1.8) | 10.3 (2.0) | 23.2 (2.5) | 16.0 (2.3) | 15.0 (2.6) | 17.1 (2.6) | 9.5 (2.2) |
Completeness (%) | 99.8 (99.0) | 91.8 (78.6) | 99.2 (99.3) | 99.1 (99.9) | 99.9 (99.9) | 99.9 (100) | 78.5 (29.2) | 93.7 (56.5) | 95.8 (97.8) |
Redundancy | 11.0 (10.9) | 3.6 (2.4) | 3.0 (3.1) | 6.8 (6.6) | 50.6 (15.1) | 14.4 (14.6) | 6.6 (5.8) | 17.0 (7.8) | 3.8 (3.0) |
Refinement | |||||||||
Resolution (Å)* | 42.59–1.60 (1.63–1.60) | 37.84–2.00 (2.05–2.00) | 37.66–2.40 (2.49–2.40) | 39.69–2.20 (2.25–2.20) | 66.46–2.80 (2.97–2.80) | 38.89–2.40 (2.49–2.40) | 65.94–2.10 (2.16–2.10) | 32.76–2.70 (2.83–2.70) | |
No. of reflections | 34,163 | 15,439 | 12,423 | 67,674 | 14,706 | 18,529 | 31,015 | 6,517 | |
Rwork/Rfree | 0.222/0.258 | 0.200/0.276 | 0.224/0.299 | 0.223/0.270 | 0.236/0.323 | 0.195/0.273 | 0.188/0.246 | 0.317/0.395 | |
No. of atoms | |||||||||
Protein | 2,121 | 2,327 | 2,220 | 6,807 | 3,967 | 3,815 | 4,145 | 1,679 | |
Water | 79 | 29 | 9 | 292 | — | — | 220 | — | |
Ligands | — | — | — | — | 1 | — | — | — | |
B-factors (Å2) | |||||||||
Protein | 41.5 | 48.6 | 64.7 | 47.9 | 86.2 | 79.8 | 35.4 | 60.4 | |
Water | 37.8 | 42.7 | 51.0 | 40.6 | — | — | 36.8 | — | |
Ligands | — | — | — | — | 79.1 | — | — | — | |
r.m.s.d. | |||||||||
Bond lengths (Å) | 0.008 | 0.013 | 0.012 | 0.012 | 0.013 | 0.015 | 0.013 | 0.004 | |
Bond angles (°) | 1.199 | 1.591 | 1.631 | 1.418 | 1.656 | 1.864 | 1.604 | 0.748 | |
Number of monomers or 1:1 complexes | 2 | 3 | 2 | 8 | 2 | 2 | 2 | 1 | |
Dimeric architecture | No | Yes | Yes | — | Yes | Yes | Yes | No | |
Protein Data Bank code | 4C84 | 4C86 | 4C8T | 4C8V | 4C99 | 4C9A | 4C9R | 4C9V | |
