Figure 8: PTB1 is responsible for the PSSR decrease mediated by abnormal temperatures.
From: Natural variation in PTB1 regulates rice seed setting rate by controlling pollen tube growth
(a) Variations in PSSR under the treatment temperatures. Values represent the mean±s.e.m. (n=3). WT (indica) and Nipponbare (japonica), together with three other ptb1–PTB1 transgenic lines with different PSSRs, S71, S64 and S55, were treated with normal, lower and higher temperatures for 5 days. (b) Relative expression levels of PTB1 under the treated temperatures. The relative expression level of the PTB1 gene in each accession was determined using q-PCR with RNA isolated from the flag leaves of each treated plant. The relative expression levels are measured as the mean±s.e.m. (n=3). Each P-value is based on a two-tailed t-test for the treatment and normal temperatures. The r value indicates the correlation between PSSRs and the relative expression levels of PTB1 under given conditions (P<0.01, Pearson correlation analysis). (c) PT growth of the WT at various temperatures. Arrows indicate the chalazal end of the embryo sac and the micropyle. Samples were collected for fluorescence microscopic observation at 2 h AP, n>30. Scale bars, 400 μm.
