Table 2 The detected C13-labelled species in shoots and roots of wild-type and atabcg14 mutant fed with C13-tZ.

From: Arabidopsis ABCG14 protein controls the acropetal translocation of root-synthesized cytokinins

Total CKs

WTSt

atabcg14St

WTRt

atabcg14Rt

[13C5]tZ

1.1±0.5

0.02±0.0*

44.2±11.1

88.8±25.3*

[13C5]tZR

6.2±2.2

0.01±0.0*

4.9±0.3***

22.5±2.3***

[13C5]tZR5′MP

n.d.

n.d.

2.1±0.5

10.8±2.7*

[13C5]tZOG

n.d.

n.d.

4.0±1.2

4.1±0.9**

[13C5]tZROG

n.d.

n.d.

0.1±0

0.2±0.0

[13C5]tZ7G

1.0±0.1

0.7±0.2

66.6±15

109.1±14.7*

[13C5]tZ9G

0.2±0

n.d.

6.4±1.2

12.4±1.6***

  1. CK, cytokinin; n.d., not detected; Rt, Root; St, Shoot; tZ, trans-zeatin; tZOG, trans-zeatin O-glucoside; tZR, trans-zeatin riboside; tZR5′ MP, trans-zeatin riboside 5′ monophosphate; tZROG, trans-zeatin riboside O-glucoside; tZ7G, trans-zeatin 7-glucoside; tZ9G, trans-zeatin 9-glucoside.
  2. Values in pmol g−1 fresh weight. Contents (means±s.d., n=4) measured in ~20 mg freeze-dried samples of roots and shoots from 10-DAG Arabidopsis seedlings fed with 5 nM [13C5]tZ for 4 h. Asterisks indicate statistically significant difference in the mutant line versus the wild-type (WT) in one-way ANOVA analysis (*, ** and *** correspond to P-values of 0.05>P>0.01, 0.01>P>0.001 and P<0.001, respectively, from t-test).
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