Figure 1: Induction of DCX⁺ cells in the adult mouse spinal cord.

(a) Experimental scheme. (b) DCX⁺ cells are detected in animals injected with lentivirus expressing SOX2 but not control GFP at 4 or 5 wpi. Nuclei were counterstained with Hoechst 33342 (Hst) (c,d) Quantification of SOX2-induced DCX⁺ cells around the virus-injected regions in adult spinal cords (mean+s.d.; n=3 mice per group; n.d., not detected). (e) Confocal images of a representative DCX⁺ cell (indicated by arrows). SOX2-induced DCX⁺ cells are traced by the coexpressed GFP, indicating an origin of virus-infected cells. They are also co-labelled by TUBB3 and have bipolar or multipolar processes. Asterisks and arrowheads indicate cell bodies and neuronal processes, respectively. (f) Representative images of DCX⁺ cells induced in the spinal cord of aged mice (>12 months). Scale bars, 50 μm (b,f) and 20 μm (e).