Figure 5: Cell proliferation during SOX2-induced neurogenesis in the adult spinal cord.

(a) Incorporation of BrdU in SOX2-induced DCX⁺ cells. An orthogonal view of BrdU⁺DCX⁺ cells in the boxed region is shown in the right panel (wk, weeks; IHC, immunohistochemistry). (b) Ki67-staining showing that SOX2-induced DCX⁺ cells are proliferative at 5 wpi. An orthogonal view of Ki67⁺DCX⁺ cells in the boxed region is shown in the right panel. (c) Quantification of BrdU or Ki67-labelled DCX⁺ cells (mean+s.d.; n=3 mice per group). (d,e) Newly generated neurons indicated by BrdU-traced TUBB3⁺ cells in the adult spinal cord. Orthogonal views of BrdU⁺TUBB3⁺ cell are also shown. (f) Quantification of SOX2-induced new neurons (indicated by BrdU⁺TUBB3⁺) surrounding the virus-injected regions in adult spinal cords (mean+s.d.; n=3 mice per group; n.d., not detected). Scale bar, 20 μm (a,b,d,e).