Figure 6: Induction of NSCs from spinal astrocytes by SOX2 in culture. | Nature Communications

Figure 6: Induction of NSCs from spinal astrocytes by SOX2 in culture.

From: In vivo conversion of astrocytes to neurons in the injured adult spinal cord

Figure 6: Induction of NSCs from spinal astrocytes by SOX2 in culture.The alternative text for this image may have been generated using AI.

(a) Experimental scheme. (b) Immunocytochemistry confirming the astrocyte identity of cultured tdT+ cells. (c) Representative micrographs of neurospheres from spinal astrocytes infected with the hGFAP-GFP-T2A-SOX2 lentivirus. (d) Frequency of neurosphere-forming cells from spinal astrocytes infected with the indicated lentivirus. Secondary spheres were quantified at 7 days after plating (mean+s.d.; n=5; *P<0.01 by Student’s t-test). (e) Nestin (NES) expression in SOX2-induced neurospheres. (f) Multiple lineage differentiation of SOX2-induced secondary neurospheres. Immunocytochemistry was performed 6 days after plating dissociated single cells. Scale bars, 50 μm (b), 200 μm (c), 60 μm (e) and 40 μm (f).

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