Figure 9: SOX2-induced neurons resemble GABAergic interneurons in the adult spinal cord. | Nature Communications

Figure 9: SOX2-induced neurons resemble GABAergic interneurons in the adult spinal cord.

From: In vivo conversion of astrocytes to neurons in the injured adult spinal cord

Figure 9: SOX2-induced neurons resemble GABAergic interneurons in the adult spinal cord.The alternative text for this image may have been generated using AI.

(a) Experimental scheme to examine SOX2-induced mature neurons. (b,c) Ectopic SOX2 converts astrocytes (indicated by tdT) to GABAergic interneurons (indicated by GABA and GAD65 expression). Coexpression of the indicated markers is also shown by an orthogonal view. These cells were not detectable in spinal cords injected with a control virus. (d) BrdU labelling indicates that SOX2-induced GABA+ cells are newly generated. Coexpression of the indicated markers is shown by an orthogonal view. (e) Expression of synapsin-I (SYN1) in tdT+ cells in spinal cords injected with SOX2 but not a control virus. An orthogonal view is to show co-localization of the indicated markers. (f,g) Confocal images showing synapse-formation between SOX2-induced neurons (labelled by tdT) and the soma (f) or axon (g) of endogenous cholinergic motoneurons (labelled by ChAT). Synapses are indicated by SYN1-expression. Scale bar, 20 μm (b–f) and 10 μm (g).

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