Figure 7: EphrinB2 and flotillin-1 morphants show neural tube closure defects.
From: EphrinB2 affects apical constriction in Xenopus embryos and is regulated by ADAM10 and flotillin-1
(a) Neural tube closure defects in ephrinB2 or flotillin-1 morphants. Dorsal view of stage 18 control, ephrinB2, flotillin-1a or flotillin-1b MO-injected embryos, and those co-injected with morpholino-resistant RNA (B211MT-HA for B2MO, F1aΔUTR-Flag for F1aMO and F1bΔUTR-Flag for F1bMO); left is anterior and right is posterior. (b) Quantification of neural tube closure defects in ephrinB2 or flotillin-1 morphants. Embryos showing a single dorsal line were counted as having closed neural tubes. At least three independent experiments were performed and error bars indicate s.d. (c) B2MO, F1aMO, or F1bMO were injected into embryos, and neural tube closure was examined at neurula (stage 18/19) and swimming tadpole stages. Note the anterior neural tube defects. (d) Percentage of neural tube closure in B2MO-, F1aMO-, or F1bMO-injected embryos. Note that the percentage of neural tube closure is somewhat elevated at later stages in B2MO-, F1aMO- and F1bMO-injected embryos, indicating that some of the embryos displayed a profound delay in neural tube closure, while others retain an open neural tube. Error bars indicate s.d. (e) EphrinB1- MO- or flotillin-2 MO-injected embryos show normal neural tube closure.
