Figure 3: Synaptic fold characterization on the TEM images from WT and transgenic mice NMJs.
From: Morphological and functional remodelling of the neuromuscular junction by skeletal muscle PGC-1α
(a) The average number of folds attached to synapses was determined for WT and MCK mice in SCM muscle. (b) Synaptic fold lengths from SCM muscles were scaled and measured with Image J and averaged. Each bar represents mean±s.e.m. (n=4, t-test two-tailed). (c) Representative TEM image of the NMJ area of SCM muscle from WT or MCK animals. (d) Western blot detection of the Nav1.4 expression in SCM muscles from WT and MCK animals. The relative band intensity was analysed using the Image J. The WT/tubulin ratio was set as 100%. (e) The average number of folds attached to synapses was determined for WT and MCK mice in soleus muscle. (f) Synaptic fold lengths from soleus muscles were scaled and measured with Image J and averaged. Each bar represents mean±s.e.m. (n=4, t-test two-tailed). SF: Synaptic folds, m: mitochondria.
