Figure 5: Electrophysiological properties of LAL muscles from WT or transgenic mice
From: Morphological and functional remodelling of the neuromuscular junction by skeletal muscle PGC-1α
(a) The spontaneous neurotransmitter release of LAL muscle resembled the representative traces of mEPP in WT (black) and MCK-PGC-1α (gray) NMJs. Amplitude, frequency of mEPP and their rise and decay times were determined. (b) Evoked neurotransmitter release resembled the representative traces of EPP in WT (black) and MCK-PGC-1α (grey) NMJs. Amplitude, quantum content of EPP and their rise and decay times were determined. (c) The synaptic plasticity shows a representative trace of steady-state depression in WT (black) and MCK-PGC-1α (grey) mice. The pair-pulse-facilitation (PPF) at inter-stimulus-intervals (ISI) ranging from 10 to 200 ms and the steady-state depression were measured at the end of the train and normalized to the amplitude of the first EPP. The normalized depression after 10 repetitive stimuli was measured between 0 and 100 Hz. The results are represented as mean±s.e.m. The numbers in the bars are n, N: number of fibres, number of mice.
