Figure 4: Mec1-dependent checkpoint signalling and homologous recombination repair are not required to overcome replication fork pausing at Tus–Ter modules. | Nature Communications

Figure 4: Mec1-dependent checkpoint signalling and homologous recombination repair are not required to overcome replication fork pausing at Tus–Ter modules.

From: The Escherichia coli Tus–Ter replication fork barrier causes site-specific DNA replication perturbation in yeast

Figure 4

(a) sml1 and sml1mec1 3xTerB ChrIII RESTRICTIVE/ChrVI RESTRICTIVE strains were released from G1-arrest and DNA replication intermediates present at ChrIII were detected by 2D gels at the indicated times. Representative results are shown from a minimum of three independent experiments. RF pausing at Tus–Ter is indicated by the black arrow. Flow cytometry data are also shown. The corresponding ChrVI data are shown in Supplementary Fig. 6. (b) Wild-type, rad50, rad51 and rad52 3xTerB ChrIII RESTRICTIVE/ChrVI RESTRICTIVE strains were analysed as described above.

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