Figure 3: Estimated efficiencies of inducing EML4-ALK and KIF5B-RET rearrangements.
From: Targeted genomic rearrangements using CRISPR/Cas technology
HEK 293T cells transfected with Cas9 and no sgRNA (vector), a single sgRNA, or a pair of sgRNAs were stained for either (a) ALK or (b) RET protein to estimate the percentage of cells with induced EML4-ALK or KIF5B-RET rearrangements, respectively. All values represent means of at least three independent experiments ±s.e.m. Means were compared by paired t-test (**P<0.01, ***P<0.001). Also shown are dotplots from a representative experiment for each rearrangement.
