Figure 4: Detection of PT by SMRT sequencing across V. cyclitrohicus FF75.
From: Genomic mapping of phosphorothioates reveals partial modification of short consensus sequences
(a) Example IPD ratio plot showing one instance of PT in a 5′-CCA-3′ sequence context. A nearby adenine methylation signal at 5′-Gm6ATC-3′/3′-CTm6AG-5′ is also shown for comparison. The absence of the PT signal, but not the methylation signal, in the XXL-1 mutant is shown in the lower panel. (b) Kinetic score distributions for the WT and XXL-1 mutant across the FF75 genome. The dashed line indicates the threshold for calling sites modified. (c) PT annotation across the FF75 genome. From outer to inner circles: 1 and 2 (forward, reverse strands): PT sites in ORFs (grey), non-coding RNA (blue) and non-encoding regions (red); 3 and 4: predicted protein-coding sequences colored according to COG function categories; 5: tRNA/rRNA operons; 6: GC content; 7: GC skew.
