Figure 7: Hoxa9 expression level is critical for transformation from MDS to AML. | Nature Communications

Figure 7: Hoxa9 expression level is critical for transformation from MDS to AML.

From: Ezh2 loss promotes development of myelodysplastic syndrome but attenuates its predisposition to leukaemic transformation

Figure 7: Hoxa9 expression level is critical for transformation from MDS to AML.The alternative text for this image may have been generated using AI.

(a) Quantitative RT–PCR analysis of the expression of HOXA9 in CD34+ cells of seven MDS–RCMD, seven MDS–RAEBI/II, five MDS/AML, four de novo AML patients, four MLL-AF9-positive AML cell lines (THP-1, NOMO-1, MOLM-13 and MONO-MAC1) and six healthy controls. Scale bars and asterisks show mean±s.d., **P<0.01, and ***P<0.001 by Mann–Whitney U-test. (b,c) Clonogenic capacity of Rx291/Ezh2Δ/Δcells overexpressing Hoxa9. WT and Rx291/Ezh2Δ/ΔHSCs were transduced with a Hoxa9 retrovirus and plated in methylcellulose media, then replated into the same medium every 14 days. The data are shown as mean±s.e.m. (n=3) in b. Expression of exogenous Flag-tagged Hoxa9 protein in colony cells was detected by western blotting using an anti-Flag antibody in c. β-Actin was detected as a loading control. (d) Morphology of AML cells from BM of recipients infused with Hoxa9-expressing Rx291/Ezh2Δ/Δ cells observed by May–Grüenwald Giemsa staining. Scale bar, 20 μm. (e) Fluorescence-activated cell sorting profiles of BM cells from mouse in d and Rx291/Ezh2Δ/Δ-MDS mouse. (f) A proposed model of the role of Ezh2 loss in the development of RUNX1S291fs-induced MDS.

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