Figure 10: Age-dependent memory loss is associated with microtubule-dependent dendritic transport of GluA2.

(a) Immunoblotting shows reduced level of total stathmin protein in the dentate gyrus (DG) of aged WT mice. n=5 per group. *P<0.05, **P<0.01 versus young adult mice (post-hoc comparison). (b) Immunoblotting shows deficiency in learning-dependent microtubule destability and hyperstability in aged mice. Detyr, detyrosinated. N, naive. n=5 per group (pooled tissues from threeto four mice per sample). *P<0.05 versus naive young adult mice (post-hoc comparison). (c–e) Immunoblot estimation of GluA2 levels in aged mice in synaptosomal (c, n=5 per group, pooled tissues from three to four mice per sample), whole cell extract (d, n=5 per group) and microtubule (e, n=5 per group, pooled tissues from 3–4 mice per sample) fractions of the DG of naïve and trained (single or three foot-shock pairings) aged and young adult mice. N, naive; #FS, number of foot-shocks; 1, one foot shock; 3, three foot shocks. *P<0.05, **P<0.01 versus naive mice in corresponding group (post-hoc comparison). (f) Intra-hippocampal injection of the TAT-GluA2_3Y peptide rescued decreased contextual fear memory in aged mice. n=12–13. *P<0.05 (post-hoc comparison). Data are expressed as mean±s.e.m.