Figure 2: Stathmin phosphorylation is essential for learning-dependent changes in microtubule stability. | Nature Communications

Figure 2: Stathmin phosphorylation is essential for learning-dependent changes in microtubule stability.

From: Learning-induced and stathmin-dependent changes in microtubule stability are critical for memory and disrupted in ageing

Figure 2

(a) Diagram illustrating transgenic design and control of transgene expression by doxycycline (dox) in Stat4A bitransgenic mice. (b) Immunohistochemistry shows that Stat4A:GFP is expressed in the DG, but not CA1 area of the hippocampus. Scale bar, 200 μm. (c) Immunoblot using anti-GFP antibody demonstrates that transgene expression is repressed by dox. (d) Western blottings from the synaptosomal fraction of the DG of Stat4A mice show a deficit in microtubule hyperstability at 8 h after contextual fear conditioning. Tyr, tyrosinated tubulin; Detyr, detyrosinated tubulin. N, naive. n=6 per group (pooled tissues from three to four mice per sample). *P<0.05 versus naive in corresponding genotype (post-hoc comparison). Data are expressed as mean±s.e.m.

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