Figure 3: Wdfy3 is upregulated in a subset of radial glia divisions during neurogenesis.

(a) Immunofluorescent detection of Wdfy3 in cortical neural progenitors positioned at the ventricle of the WT cerebral cortex at E14.5 illustrates upregulation in dividing cells throughout all stages of mitosis. (b) Wdfy3 coexpression analysis with Pax6, a marker of radial glia and Tbr2, a marker of intermediate progenitor. Wdfy3⁺ cells present only a subset of dividing progenitors (red arrowheads) and are surrounded by Wdfy3⁻ mitotic cells. (c) Coexpression analysis of Wdfy3 with the mitosis marker phospho-histone H3⁺ (pHH3) confirms that only a small share of mitotic cells is Wdfy3⁺. (d) Quantification of Wdfy3⁺/pHH3⁺ cells at E12.5 and E14.5 shows significant reductions in the disc/disc mutants. Analysis by Student’s t-test. Bar diagrams show mean and s.e.m., number of analysed embryos (n) and calculated P values are indicated in the diagrams. Scale bar in a is 10 μm, in b 20 μm and in c 40 μm.