Figure 1: Characterization of PSD-95-GFP-(+) and (−) spines.
From: Enhanced synapse remodelling as a common phenotype in mouse models of autism
(a) Postnatal development of dendritic spines and PSD-95 clusters in wild-type mice. Imaging of PSD-95-GFP (green) and DsRed2 (grey) revealed PSD-95-GFP-(+) (arrows) and PSD-95-GFP-(−) spines (arrowheads) in the SSC at different time points of postnatal development. (b) Postnatal increase of total spines and spines positive with PSD-95-GFP clusters in the SSC of wild-type mice. Error bars indicate s.e.m. of the spine densities classified as PSD-95-GFP-(+) or PSD-95-GFP-(−). (c) Turnover rates of total spines at different time points. (d) Distribution of spine volumes classified by the presence or absence of PSD-95-GFP clusters (PSD-95-(+) spines, n=353; PSD-95-(−) spines, n=43). (e) In vivo imaging of PSD-95-GFP (grey in middle panels, green in right panels) and dendrites filled with DsRed2 (grey). Gain (arrows) and loss (arrowheads) of PSD-95-GFP-(+) spines were detected. (f) Gain and loss of spines classified by the presence or absence of PSD-95-GFP clusters at three different time points during postnatal development in wild-type mice. Data are presented as mean±s.e.m. *P<0.05, ***P<0.005, one-way analysis of variance, Tukey’s-test. Refer to the Supplementary Table 1 for the number of animals in each group. Scale bars, 2 μm.
