Figure 4: patDp/+ mice show enhanced spine dynamics in the AFC at postnatal week 3 and in the SSC at postnatal week 2.
From: Enhanced synapse remodelling as a common phenotype in mouse models of autism
(a) In vivo time-lapse imaging of PSD-95-GFP (green) and dendrites filled with DsRed2 (grey) over 2 days in the AFC of wild-type and patDp/+ mice. Gain (arrow) and loss (arrowhead) of PSD-95-GFP-(+) spines were detected. (b) Spine density with or without PSD-95-GFP clusters in the AFC of wild-type and patDp/+ mice. Error bars indicate the s.e.m. of the spine densities classified as PSD-95-GFP-(+) or PSD-95-GFP-(−). (c) Turnover of PSD-95-GFP-(+) and (−) spines over 2 days in the AFC of wild-type and patDp/+ mice at postnatal week 3. (d) Gain and loss of PSD-95-GFP-(+) and (−) spines per unit length of dendrites in the AFC of wild-type and patDp/+ mice at postnatal week 3. (e) In vivo time-lapse imaging of PSD-95-GFP (green) and dendrites filled with DsRed2 (grey) in the SSC of wild-type and patDp/+ mice at postnatal week 2. Gain (arrows) and loss (arrowhead) of spines were detected. (f) Spine density with or without PSD-95-GFP clusters in the SSC of wild-type and patDp/+ mice at postnatal week 2. Error bars indicate the s.e.m. of the spine densities classified as PSD-95-GFP-(+) or PSD-95-GFP-(−). (g) Turnover of PSD-95-GFP-(+) and (−) spines over 1 day in the SSC of wild-type and patDp/+ mice at postnatal week 2. (h) Gain and loss of PSD-95-GFP-(+) and (−) spines per unit length of dendrites in the SSC of wild-type and patDp/+ mice at postnatal week 2. Data are presented as mean±s.e.m. *P<0.05, ***P<0.005, Student’s t-test. Refer to the Supplementary Table 2 for the number of animals in each group. Scale bars, 3 μm.
