Figure 4: BIND biofilms can be programmed to adhere to specific substrates.
From: Programmable biofilm-based materials from engineered curli nanofibres
(a) Adhesion of PHL628 ΔcsgA cells producing no CsgA (left), wild-type CsgA (middle) and CsgA-MBD (right) was tested by spotting induced cultures onto a 304L steel coupon and incubating for 48 h at 4 °C. The ring formation is due to cells being drawn to the edges of the droplet during drying, known as the ‘coffee ring effect’. (b)The MBD-BIND biofilms were analysed by FE-SEM. Scale bar is 250 μm. (c) Magnification of the boxed area in b of a false-coloured FE-SEM showing MBD-BIND cells adhered to the 304L surface. Scale bar is 10 μm. (d) Magnification of the boxed area in c of a false-coloured FE-SEM showing a zoomed-in view of the cell bodies. Because of the vigorous washing process, some of the cell bodies appear damaged. Scale bar is 2 μm.
