Figure 4: De novo truncating TBR1 mutations disrupt interactions with CASK.
From: De novo TBR1 mutations in sporadic autism disrupt protein functions
Fluorescence micrographs of HEK293 cells co-transfected with CASK and (a) WT TBR1, (b) de novo truncating variants and (c) de novo missense variants. Xpress-tagged TBR1 proteins are shown in red (left-hand side), whereas CASK fused to YFP is shown in green (middle). Nuclei were stained with Hoechst 33342 (blue). Scale bars for a–c=10 μm. Concordant results were seen in SHSY5Y cells, as shown in Supplementary Fig. 4.
