Figure 4: TG-induced H2O2 is produced by the mitochondrial respiratory chain.
From: Red fluorescent genetically encoded indicator for intracellular hydrogen peroxide
(a) Effects of Ca2+-free media and rotenone on the intensity of H2O2 increase in mitochondria. The bars show normalized values±s.d., 25 cells from two experiments for Ca2+-free media, 42 cells from three experiments for rotenone. (b) Timing of mitochondrial H2O2 production (HyPerRed-mito) compared with mitochondrial Ca2+ elevation (B-GECO-mito). The curves were ‘synchronized’ by the TG addition time point (1 min). The inset shows the moment when mitochondrial Ca2+ starts increasing before H2O2.
