Figure 3: Some fALS SOD1 mutants behave like WT SOD1.
From: In-cell NMR reveals potential precursor of toxic species from SOD1 fALS mutants
1H–15N SOFAST HMQC spectra acquired on human cell samples expressing uniformly 15N-labelled WT SOD1 and mutants in zinc-supplemented medium. A subset of the fALS-linked SOD1 mutants behave like WT SOD1, and stoichiometrically bind one zinc ion per monomer, when present in excess in the cell culture. The very small chemical shift differences between WT and mutant SOD1 indicate high structural similarity (see Supplementary Fig. 4).
