Figure 6: The unstructured form of apo-G93A^SH does not have any β-strand structural propensity.

Correlation plots between experimental and predicted N (a) and Cα (b) chemical shifts using random coil libraries⁵⁶ are reported for the unstructured apo-G93A^SH, the native, folded apo WT SOD1^SH and the fully mature, oxidized Cu(I),Zn-SOD1^SS. The fit parameters for the function f(x)=ax+b using least-square method (R² and root mean square difference (RMSD)) are indicated for each plot; c) the overall secondary structure propensity (SSP) score per residue obtained by using a weighted combination of backbone secondary chemical shifts⁵⁷ is reported for unstructured apo-G93A^SH, apo WT SOD1^SH and Cu(I),Zn-SOD1^SS. Positive SSP values indicate α-helical propensity while negative values correspond to β-strand propensity.