Figure 5: Detection of IDO in monocytes. | Nature Communications

Figure 5: Detection of IDO in monocytes.

From: High-throughput detection of miRNAs and gene-specific mRNA at the single-cell level by flow cytometry

Figure 5

PBMCs from an HIV-negative subject were stimulated for 8 h with either R848 or IFNγ+LPS. IDO mRNA detection in monocytes was achieved after gating on the CD14 positive cell subset within the monocyte gate. (a) Representative example and (b) collective data on five different subjects. Statistical analysis used Friedman test with a Dunn’s post-test comparison. (c) Correlation between IDO mRNA mean fluorescent intensity (MFI) in CD14+ cells and IDO relative mRNA expression as measured by qPCR in PBMCs from the same experiment. Statistical analysis used the Spearman rank sum test.

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