Figure 3: Effect of Leu206Arg and Leu199_Cys200insTrp mutations on the retention of Cα subunits on DEAE-Sephacel. | Nature Communications

Figure 3: Effect of Leu206Arg and Leu199_Cys200insTrp mutations on the retention of Cα subunits on DEAE-Sephacel.

From: PKA catalytic subunit mutations in adrenocortical Cushing’s adenoma impair association with the regulatory subunit

Figure 3: Effect of Leu206Arg and Leu199_Cys200insTrp mutations on the retention of Cα subunits on DEAE-Sephacel.

(a–c) Results of experiments where the lysates of cells co-transfected with R and C subunits were pre-bound to DEAE-Sephacel columns, which have a high affinity for R subunits, and eluted with wash buffer followed by the same buffer containing increasing concentrations of cAMP. Data are from 4 independent experiments per condition. (a) Western blot analysis for Cα. Shown are three representative blots. (b) PKA activity (mean±s.e.m.) measured in the different fractions (n=4). (c) Comparison of the fraction of PKA activity (mean±s.e.m.) collected during the first two washes with buffer (n=4). *P<0.05 versus WT by one-way ANOVA followed by Bonferroni’s post-hoc test.

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