Figure 3: Knockdown of PKCε causes cells to enter anaphase prematurely when challenged by catenation.
From: Mitotic catenation is monitored and resolved by a PKCε-regulated pathway
The fidelity of the metaphase catenation arrest was assessed by time-lapse microscopy. (a,b) HeLa-H2B cells were treated for 48 h±siRNA targeting PKCε. Cumulative frequency chart (a) and representative stills, time in minutes marked in white (b) showing time taken in minutes from metaphase-to-anaphase±5 μM ICRF193. (c,d) Inhibition of PKCε M486A in DLD-1 cells abrogates the metaphase catenation delay as illustrated by cumulative frequency chart (c) and representative stills (d), showing time taken in minutes from metaphase to anaphase after treatment with NaPP1±5 μM ICRF193. (e–g) HeLa-H2B cells were treated with either control siRNA or one of three different siRNAs that target PKCε (si1, si2 and si3). (e,f) Cumulative frequency charts showing the time spent in either metaphase (e) or prometaphase (f), and representative stills from the time-lapse videos, time in minutes marked in white (g). For all live-cell experiments, n>30, all experiments repeated three times. Scale bars, 5 μm.
