Figure 4: Knockdown of PKCε using siRNA does not affect the SAC arrest in DLD-1 cells.
From: Mitotic catenation is monitored and resolved by a PKCε-regulated pathway
(a–d) DLD-1 cells were treated with various SAC triggers and the fidelity of the SAC arrest was measured after loss of PKCε. (a,b) DLD-1 parental cells or DLD-1 PKCε M486A cells were treated with 100 μM Monastrol±20 nM NaPP1 (a) or with PKCε si1 (b) and time taken to transit through mitosis was assayed by time-lapse microscopy by monitoring cell rounding. Charts show the number of cells that maintain a mitotic arrest for more than 7 h. (c,d) DLD-1 parental cells or DLD-1 PKCε M486A cells were treated with taxol or ICRF193 as indicated±20 nM NaPP1 or with PKCε si1 and the time taken to transit through mitosis was assayed by time-lapse video microscopy by monitoring cell rounding. The graph shows the time taken to transit through mitosis as a cumulative frequency chart. For all live-cell experiments, n>30, all experiments repeated three times.
