Figure 1: Pattern of H2AX phosphorylation in scratched monolayers of fetal NHAs. | Nature Communications

Figure 1: Pattern of H2AX phosphorylation in scratched monolayers of fetal NHAs.

From: Cell transcriptional state alters genomic patterns of DNA double-strand break repair in human astrocytes

Figure 1

(a) Fetal NHA monolayers were grown to confluence over 10 days, scratched with a pipette tip and fixed 24 h later. Monolayers were co-stained for GFAP and γH2AX, and counterstained with 4′,6-diamidino-2-phenylindole (DAPI). (b) Same as in a, but monolayers were co-stained for nestin and γH2AX, and counterstained with DAPI. (c) Same as in a, but monolayers were irradiated with 5 Gy X-rays 24 h after scratch and fixed 30 min later. (d) Same as in b, but monolayers were irradiated with 5 Gy X-rays 24 h after scratch and fixed 30 min later. Arrowheads indicate scratch edge and direction of cell migration. Scale bars, 100 μm.

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