Figure 4: ChIP-qPCR analysis of confluent and scratched fetal NHAs. | Nature Communications

Figure 4: ChIP-qPCR analysis of confluent and scratched fetal NHAs.

From: Cell transcriptional state alters genomic patterns of DNA double-strand break repair in human astrocytes

Figure 4

(a) Cross-linked chromatin from confluent or scratched monolayers of fetal NHAs was immunoprecipitated using normal rabbit IgG, or antibodies against histone H3 or γH2AX, resolved using SDS–PAGE and immunoblotted for γH2AX. (b) Representative qPCR analysis of six gene loci using chromatin from irradiated confluent fetal NHAs. See Supplementary Table 2 for primers used. (c) Same as in b, except chromatin from irradiated scratched fetal NHAs was used. (d) Mean scratch-to-confluent ratio of normalized γH2AX qPCR signals for loci exhibiting high rates of somatic mutation in GBM. Bars are s.e. for four biological replicates, with each replicate consisting of the average of technical duplicates. (e) Same as in d for loci overexpressed in scratched compared with confluent fetal NHAs.

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