Figure 1: Screening animals for ZFN-targeted mutation at the Nr2f2 locus. | Nature Communications

Figure 1: Screening animals for ZFN-targeted mutation at the Nr2f2 locus.

From: Mutation within the hinge region of the transcription factor Nr2f2 attenuates salt-sensitive hypertension

Figure 1

(a) Tail DNA samples from pups born post microinjection of custom ZFNs targeting exon 2 of the Nr2f2 locus were screened by PCR amplification with primers designed to amplify rat genomic fragments encompassing the ZFN-targeted site. Deviations from the expected product size of 360 bp were inferred as genomic DNA from rats with mutations at the Nr2f2 locus. (b) Representative sequencing results from the PCR products shown in a detected a 15 bp deletion in the mRNA from mutant rats. Also represented are the corresponding translated peptide sequences as a result of the nucleotide variations.

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