Figure 3: PD-1 induces upregulation of CPT1A and fatty acid β-oxidation. | Nature Communications

Figure 3: PD-1 induces upregulation of CPT1A and fatty acid β-oxidation.

From: PD-1 alters T-cell metabolic reprogramming by inhibiting glycolysis and promoting lipolysis and fatty acid oxidation

Figure 3: PD-1 induces upregulation of CPT1A and fatty acid β-oxidation.

(a) CPT1A mRNA was analysed by real-time quantitative PCR and relative expression of mRNA of each time point and culture condition over the levels expressed in unstimulated cells (defined as 1) is shown (*P<0.05, TCC+PD1 versus TCC, Student’s t-test; ♦P<0.05, TCC+PD1 versus UT and TCC versus UT, analysis of variance (ANOVA); n=3 experiments). (b) CD4+ primary human T cells were cultured under the indicated conditions. Cell lysates were prepared at the indicated time points and expression of CPT1A and β-actin were assessed by SDS–PAGE and immunoblot. Results are representative of three experiments. (c) Fatty acid β-oxidation rate after culture under the indicated conditions for various time intervals was examined. Values of TCC and TCC+PD1 cells were compared with unstimulated (UT) cells (♦P<0.05, ANOVA; n=3) and values of TCC+PD1 were compared with TCC cells (*P<0.05, Student’s t-test; n=3).

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