Figure 5: T cells receiving PD-1 signals have substantial mitochondrial spare respiratory capacity. | Nature Communications

Figure 5: T cells receiving PD-1 signals have substantial mitochondrial spare respiratory capacity.

From: PD-1 alters T-cell metabolic reprogramming by inhibiting glycolysis and promoting lipolysis and fatty acid oxidation

Figure 5: T cells receiving PD-1 signals have substantial mitochondrial spare respiratory capacity.

(a,b) CD4+ primary human T cells were cultured under the indicated conditions and 72 h of culture extracellular acidification rate (ECAR) and oxygen consumption rates (OCR) were assessed. (c) OCR/ECAR ratio was also measured. (d) OCR for each group was measured in real time under basal conditions and after addition of the indicated mitochondrial inhibitors. (e) Spare respiratory capacity (SRC) indicated by the difference of maximum OCR over basal OCR (arrows in d) calculated as percentage of basal OCR was also determined. Values of TCC and TCC+PD1 cells were compared with unstimulated (UT) cells (♦P<0.05, analysis of variance; n=4); values of TCC+PD1 cells were compared with TCC cells (*P<0.05, Student’s t-test; n=4).

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