Figure 3: FRET changes during translocation.
From: Fluctuations between multiple EF-G-induced chimeric tRNA states during translocation on the ribosome
Data are displayed as contour plots after post synchronization. Fluorescence intensities and FRET trajectories are shown in Supplementary Fig. 2. Population distributions between the states and transition rates are given in Table 1. (a) After addition of EF-G(wt)–GTP. (b) EF-G(XL)–GTP. The FRET efficiency of the intermediate state was 0.40±0.09. (c) EF-G(XL) was activated by adding 2-mercaptoethanol (2-ME), while bound to the ribosome in the FRET 0.4 state. (d) EF-G(XL) was activated by 2-ME before binding to the ribosome. (e) EF-G(wt) with GTPγS. (f) EF-G(Δ4/5). (g) EF-G(H91A). (h) EF-G(H583K). (i) EF-G(wt) with Fus.
